Leukocyte changes during acute cross-circulation experiments between leukemic and normal or irradiated rats.

TVitIi the technical assistance of MARY CHALTAS T HI’ viability amid fate of circulating and tramisfused mammalian leukocytes are poorly understood. In order to investigate sonic of these problems more completely high leukocyte coumit (leukemic) domior rat-s were cross-circulated with normal and leukopemiic recipiemits. Post cross-circulatiomi hematologic observations its these animals imidicate that donor-recipient leukocyte concentratiomi relatiomiships may be an important determinant of blood leukocyte survival. Leukopenic rats retaims beukocytes in their peripheral blood for very short periods of time folbowimig cross-circulation with msormal animals.’ There is good evidemice, however, thatthe cross-transfused beukocytes remain viable its mioncirculatitig sites for much bomiger periods of time.’ 2 These data suggest that the blood survival time of traissfused leukocyt-es might be imicreased if the “pool” of tissue leukocytes was normally saturated, amid acted to impede circulatory loss. Its order to test this hypothesis attempt-s first were made to “saturate” leukopetsic animals with large volumes of leukocytes in order to slow the rate of peripheral leukocyte removal. Secondly, the peripheral leukocyt-e counts of normisl rats were elevated greatly through cross-circulation with leukemic rats in order to demonstrate the ability of normally saturated reserves to affect more prolomiged circulation of the transfused cells.